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| Bach,E.E.; Alcântara,V.B.G.; Alcântara,P.B.; Veasey,E.A.. |
| This study characterized seven Pennisetum purpureum varieties, namely v. Anão, Bajra, Cameroon, Guaçu, Roxo, Taiwan A-144 and Uruckwami, through biochemical analyses, including protein, glucose and fructose contents, and polyacrylamide gel electrophoresis using the esterase system, by sampling 30, 60, 90, 120 and 150 day-old leaves. The number of nodes per stem and the percentage of bud emergence were also recorded. Variety Taiwan A-144 presented the highest number of nodes per stem and percentage of emerging buds. Protein concentration decreased gradually after 60 days for all varieties, except for Anão. Variety Guaçu presented the highest level of glucose in 90 day-old plants, whereas Cameroon presented the highest levels at 120 and 150 days. The... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Electrophoresis; Reducing sugars; Proteins; Elephant grass; Pennisetum purpureum. |
| Ano: 1995 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90161995000300019 |
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| Baldassi,L.; Barbosa,M.L.; Bach,E.E.; Iaria,S.T.. |
| Eighty-nine of 144 isolates of Clostridium perfringens obtained from 187 samples of 71 bovine in several Brazilian states were submitted to esterase electrophoresis for typing. Mobilites electrophoresis, as parameter, were settled down by isolates from ATCC pattern of types A, B, C, and D. Of the 89 isolates, 43 (48.3%) were characterized as electrophoretic type A, 20 (22.5%) as D, 18 (20.2%) as C, and 3 (3.4%) as B. Five (5.6%) isolates did not identity with any type. Similarly, esterase electrophoresis enabled the typing of 94.4 % of the isolates, demonstrating to be an appropriate method for animal sample analyses. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Clostridium perfringens; Bovine; Electrophoresis; Esterase. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992003000200009 |
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| Baldassi,L.; Barbosa,M.L.; Bach,E.E.; Iaria,S.T.. |
| Ten out of eighty-nine strains biochemically identified as Clostridium perfringens, isolated from bovine organs, were selected by their different results showed in toxigenicity test on mice. Those and the standard strains, ATCC types A, B, C, and D, had their virulence exalted through serial intramuscular inoculation into guinea pigs. Results showed that, for toxigenic strains (6), one or two passages were enough to cause exaltation, while for the atoxigenic (4), five or six inoculations were needed. Esterase electrophoresis of standard and isolated strains, with and without exaltation, was performed. Electrophoresis analysis permits the following conclusions: strains that do not show any clinical symptoms in mice, when exalted demonstrate decreased... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Clostridium perfringens; Bovine; Electrophoresis; Virulence exaltation. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992004000300007 |
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